WB
Loading: 2 μg total protein from Arabidopsis / rice leaf Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-Actin rabbit polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of full length actin-1 from Arabidopsis AT2G37620. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | AT2G37620， LOC_Os03g61970 |
|---|---|
| Alternative Names | Actin Act |
| Molecular Weight(MW) | 42 / 42 kDa forArabidopsis thaliana42 / 42 kDa for rice |
| Function | Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms.. |
WB
Loading: 2 μg total protein from Arabidopsis / rice leaf Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
