WB
1. 0.1 μg E. coli lysate supernatant (without GST tag) 2. 0.1 μg E. coli lysate supernatant (with GST recombinant plasmid, before induction) 3. 0.1 μg E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) 4. 10 ng E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) 5. 2 ng E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) 6. 1 ng E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) Loading: E. coli lysate supernatant Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GST-tag Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein containing a sequence corresponding to amino acids 1-210 of GST protein (roughly 25 kDa) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Function | Anti-GST-tag rabbit antibody recognizes GST, a widely used tag in protein expression applications. This antibody recognizes both-C- and N-terminal GST and is especially suited to Western blot applications. |
|---|
WB
1. 0.1 μg E. coli lysate supernatant (without GST tag) 2. 0.1 μg E. coli lysate supernatant (with GST recombinant plasmid, before induction) 3. 0.1 μg E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) 4. 10 ng E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) 5. 2 ng E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) 6. 1 ng E. coli lysate supernatant (with GST recombinant plasmid, after 2 h induction) Loading: E. coli lysate supernatant Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
