WB
oading: E. coli lysate supernatant Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted 1:5000, incubated at room temperature for 1 h| Product Name | Anti-Strep-Tag II Rabbit Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide corresponding to Strep-tag II（WSHPQFEK）. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Function | Anti-Strep-Tag II rabbit antibodyrecognizes Strep-tag II, a widely used tag in protein expression applications. This antibody recognizes both-C- and N-terminal Strep-tag II and is especially suited to Western blot applications. |
|---|
WB
oading: E. coli lysate supernatant Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
