WB
Loading: 2 μg total protein from Arabidopsis / rice leaf
Electrophoresis: 12.5% SDS-PAGE
Transfer: NC (nitrocellulose) membrane, 10 V overnight
Blocking: 5% skim milk, incubated at room temperature for 1 h
Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h