WB
Loading: Serially diluted antigen protein and approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-MPK1/MAPK6/ Mitogen-activated protein kinase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Rice LOC_Os06g06090，Q84UI5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | LOC_Os06g06090，Os06g0154500 |
|---|---|
| Alternative Names | MPK1 MAPK6 SIPK |
| Molecular Weight(MW) | 44.9 kDa for Rice |
| Function | MAPK6 encodes a MAP kinase induced by pathogens, ethylene biosynthesis, oxidative stress and osmotic stress. |
WB
Loading: Serially diluted antigen protein and approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
