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Loading: Approximately 6 μg total protein from maize and rice grains Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-SU1 / Isoamylase SU1 Rabbit Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Maize Zm00001eb174590 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | RiceMaize |
| Gene Symbol | Zm00001eb174590，GRMZM2G138060 |
|---|---|
| Alternative Names | Protein SUGARY 1 |
| Molecular Weight(MW) | 88 / 83 kDa for Maize |
| Function | Isoamylase SU1 starch-debranching enzyme is involved in amylopectin biosynthesis in endosperm. |
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Loading: Approximately 6 μg total protein from maize and rice grains Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
