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Loading: Approximately 6 μg total protein from maize B73 and rice grains Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-Sh2 / Shrunken2 / ADP-glucose pyrophosphorylase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Maize Zm00001eb159060 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | RiceMaize |
| Gene Symbol | Zm00001eb159060，GRMZM2G429899 |
|---|---|
| Alternative Names | ADP-glucose synthase，AGPase S |
| Molecular Weight(MW) | 74 / 54 kDa for Maize |
| Function | Sh2 is glucose-1-phosphate adenylyltransferase large subunit 1, chloroplastic/amyloplastic; This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP- glucose from Glc-1-P and ATP. |
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Loading: Approximately 6 μg total protein from maize B73 and rice grains Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
