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Loading: Approximately 6 μg total protein from maize and rice grains Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-SBE1/ Starch-branching enzyme |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Maize Zm00001eb228530 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | RiceMaize |
| Gene Symbol | Zm00001eb228530, GRMZM2G088753 |
|---|---|
| Alternative Names | Q-enzyme 1 4-alpha-glucan-branching enzyme 2 SBEI |
| Molecular Weight(MW) | 90.5 / 93 kDa for Maize and Rice |
| Function | Sbe1 catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. |
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Loading: Approximately 6 μg total protein from maize and rice grains Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
