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Loading: 1 μg total protein from Arabidopsis / rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-NDHS / NAD(P)H-quinone oxidoreductase subunit S |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of rice LOC_Os07g09800. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | LOC_Os07g09800 |
|---|---|
| Alternative Names | NDHS NAD(P)H-quinone oxidoreductase subunit S. |
| Molecular Weight(MW) | 26 / 26 kDa forRice |
| Function | NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient (Probable). Required for the efficient operation of ferredoxin-dependent plastoquinone reduction. Forms the electron donor-binding subcomplex in association with the NDHT and NDHU subunits. |
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Loading: 1 μg total protein from Arabidopsis / rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:5000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
