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Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-su4/ISA2/ sugary4 rabbit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Maize GRMZM2G090905 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | GRMZM2G090905，Zm00001d038121 |
|---|---|
| Alternative Names | ISA2，DBE1，Isoamylase 2，BE2，Su4 |
| Molecular Weight(MW) | 79 kDa for Maize |
| Function | Starch synthesis. |
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Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
