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Loading: Total protein from rice leaves, loaded at 0.5 μg, 1 μg, and 2 μg sequentially from left to right Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GID2 / Gibberellin receptor GID2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Rice LOC_Os02g36974. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000-1:10000 |
|
| Species Reactivity | Rice |
| Gene Symbol | LOC_Os02g36974 |
|---|---|
| Alternative Names | 14-3-3-like protein GF14-E |
| Molecular Weight(MW) | 30 kDa forRice |
| Function | GID2 is essential component of some SCF-type E3 ligase complex that positively regulates the gibberellin signaling pathway. Upon gibberellin treatment, the complex mediates the ubiquitination and subsequent degradation of DELLA protein SLR1, a repressor of the gibberellin pathway, leading to activate the pathway. |
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Loading: Total protein from rice leaves, loaded at 0.5 μg, 1 μg, and 2 μg sequentially from left to right Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:5000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
