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Loading: Total protein from rice leaves at 0.5 μg, 1 μg, and 2 μg sequentially from left to right Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GID1 / Gibberellin receptor GID1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Rice LOC_Os05g33730. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000-1:10000 |
|
| Species Reactivity | Rice |
| Gene Symbol | LOC_Os05g33730 |
|---|---|
| Alternative Names | gibberellin receptor GID1L2 |
| Molecular Weight(MW) | 40 / 40 kDa forRice |
| Function | GID1 functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active GAs such as GA1, GA3 and GA4, but has low or no affinity for the biologically inactive GAs. Upon GA-binding, it interacts with the DELLA protein SLR1, a repressor of GA signaling. This leads to SLR1 degradation by the proteasome, allowing the GA signaling pathway. |
wb
Loading: Total protein from rice leaves at 0.5 μg, 1 μg, and 2 μg sequentially from left to right Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:5000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
