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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves, as well as serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-AGY1/ chloroplast protein-transporting ATPase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT4G01800，F4JG57 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT4G01800 |
|---|---|
| Alternative Names | AGY1 Albino or Glassy Yellow 1 Arabidopsis thaliana chloroplast SecA AtcpSecA SECA1 |
| Molecular Weight(MW) | 117.4 kDa forArabidopsis thaliana |
| Function | AGY1 encodes the ATPase subunit of the chloroplast Sec translocation machinery which plays an essential role in chloroplast biogenesis and the regulation of photosynthesis, the absence of which triggers a retrograde signal, eventually leading to a reprogramming of chloroplast and mitochondrial gene expression. |
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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves, as well as serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
