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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-PGR5/ Proton gradient regulation 5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT2G05620，Q9SL05 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT2G05620 |
|---|---|
| Alternative Names | ATPGR5，PGR5，proton gradient regulation 5，Protein PROTON GRADIENT REGULATION 5 chloroplastic |
| Molecular Weight(MW) | 14.3 kDa forArabidopsis thaliana |
| Function | PGR5 is involved in electron flow in Photosystem I. It is essential for photoprotection. |
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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
