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Loading: Approximately 3 μg total protein from Arabidopsis leaves and serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-BCCP1/Biotin carboxyl carrier protein of acetyl-CoA car |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G16390，Q42533 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Gene Symbol | AT5G16390 |
|---|---|
| Alternative Names | BCCP BCCP-1 BCCP1 BIOTIN CARBOXYL CARRIER PROTEIN BIOTIN CARBOXYL-CARRIER PROTEIN 1 CAC1 CAC1-A CAC1A chloroplastic acetylcoenzyme A |
| Molecular Weight(MW) | 29.6 kDa forArabidopsis thaliana |
| Function | This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA. |
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Loading: Approximately 3 μg total protein from Arabidopsis leaves and serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
