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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-BIN2/ BRASSINOSTEROID-INSENSITIVE 2 |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT4G18710，Q39011 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT4G18710 |
|---|---|
| Alternative Names | ATSK21 BIN2 DWARF 12 DWF12 SHAGGY-LIKE KINASE 21 SK21 UCU1 ULTRACURVATA 1 |
| Molecular Weight(MW) | 43 kDa forArabidopsis thaliana |
| Function | Encodes BIN2, a member of the ATSK (shaggy-like kinase) family. BIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. |
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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
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| Form | Liquid |
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| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
