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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-CHC1/ Clathrin heavy chain 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT3G11130，Q0WNJ6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT3G11130 |
|---|---|
| Alternative Names | ATCHC1 CHC1 CLATHRIN HEAVY CHAIN 1 HAS1 hot ABA deficiency suppressor 1 |
| Molecular Weight(MW) | 193 kDa forArabidopsis thaliana |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.CHC1 heavy chain subunit of clathrin，is involved in vesicle mediated trafficking. |
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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
