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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-EFE/ Ethylene-forming enzyme |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G05010，Q06588 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT1G05010 |
|---|---|
| Alternative Names | ACC oxidase，1-aminocyclopropane-1-carboxylate oxidase 4，ACO4，ACO1 EAT1，EFE |
| Molecular Weight(MW) | 36.7 kDa forArabidopsis thaliana |
| Function | EFE encodes 1-aminocyclopropane-1-carboxylate oxidase involved in the ethylene biosynthesis. |
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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
