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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-SAM2/ S-adenosylmethionine synthase 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT4G01850，P17562 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT4G01850 |
|---|---|
| Alternative Names | AtSAM2 MAT2 S-ADENOSYLMETHIONINE SYNTHETASE 2 SAM-2 SAM2 SAMS2 |
| Molecular Weight(MW) | 43.3 kDa forArabidopsis thaliana |
| Function | SAM2 encodes an S-adenosylmethionine synthase that catalyzes the formation of S-adenosylmethionine from methionine and ATP. It functions in ethylene biosynthetic pathway. |
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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
