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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-VHA-E1/ V-type proton ATPase subunit E1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT4G11150，Q39258 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT4G11150 |
|---|---|
| Alternative Names | TUF TUFF VACUOLAR ATP SYNTHASE SUBUNIT E1 VHA-E1 VHAE1 emb2448 embryo defective 2448 |
| Molecular Weight(MW) | 26 kDa forArabidopsis thaliana |
| Function | VHAE1 encodes a vacuolar H+-ATPase subunit E isoform 1 which is required for Golgi organization and vacuole function in embryogenesis. |
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Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
