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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-SRK2I/ Serine/threonine-protein kinase SRK2I |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G66880，Q39193 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT5G66880 |
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| Alternative Names | PROTEIN KINASE 2-3 SUCROSE NONFERMENTING 1(SNF1)-RELATED PROTEIN KINASE 2.3 |
| Molecular Weight(MW) | 41 kDa forArabidopsis thaliana |
| Function | SRK2I encodes a member of SNF1-related protein kinases (SnRK2) whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. It is involved in the ABA signaling during seed germination, dormancy and seedling growth. |
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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
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| Form | Liquid |
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| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
