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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane at 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000 and incubated at room temperature for 1 h| Product Name | Anti-FLS2/ LRR receptor-like serine/threonine-protein kinase |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G46330，Q9FL28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT5G46330 |
|---|---|
| Alternative Names | FLAGELLIN-SENSITIVE 2 FLS2 MPL12.8 |
| Molecular Weight(MW) | 129 kDa forArabidopsis thaliana |
| Function | FLS2 encodes a leucine-rich repeat serine/threonine protein kinase that is expressed ubiquitously. FLS2 is involved in MAP kinase signalling relay involved in innate immunity. It is essential in the perception of flagellin, a potent elicitor of the defense response. |
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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane at 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000 and incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
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| Form | Liquid |
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| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
