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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-VRN1/ B3 domain-containing transcription factor VRN1 |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT3G18990，Q8L3W1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT3G18990 |
|---|---|
| Alternative Names | REDUCED VERNALIZATION RESPONSE 1 REM39 REPRODUCTIVE MERISTEM 39 VRN1 |
| Molecular Weight(MW) | 39.3 kDa forArabidopsis thaliana |
| Function | VRN1 is required for vernalization. It is essential for the complete repression of FLC in vernalized plants. It is required for the methylation of histone H3. |
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Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
