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Loading: Approximately 3 μg of total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GC6/ Golgin candidate 6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT3G27530，B0F9L4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT3G27530 |
|---|---|
| Alternative Names | GC6 GOLGIN CANDIDATE 6 MAG4 MAIGO 4 |
| Molecular Weight(MW) | 101.8 kDa forArabidopsis thaliana102.3 kDa for Rice |
| Function | Golgi matrix protein plays a role in tethering of vesicles to Golgi membranes and maintains the overall structure of the Golgi apparatus. It functions in the anterograde transport of storage protein precursors from the endoplasmic reticulum (ER) to the Golgi complex. |
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Loading: Approximately 3 μg of total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
