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Loading: Approximately 3 μg of total protein from Arabidopsis leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GLT1/ Glutamate synthase 1 [NADH], chloroplastic |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G53460，Q9LV03 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Gene Symbol | AT5G53460 |
|---|---|
| Alternative Names | GLT1 NADH-DEPENDENT GLUTAMATE SYNTHASE 1，NADH-dependent glutamate synthase 1 (NADH-GOGAT 1) |
| Molecular Weight(MW) | 242 / 236.5 kDa forArabidopsis thaliana |
| Function | NADH-dependent glutamate synthase，is involved in glutamate biosynthesis. It is required for non-photorespiratory ammonium assimilation. |
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Loading: Approximately 3 μg of total protein from Arabidopsis leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
