WB
Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-UBA1/ Ubiquitin-activating enzyme E1 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT2G30110，P93028 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT2G30110 |
|---|---|
| Alternative Names | ATUBA1 MODIFIER OF SNC1 5 MOS5 UBA1 UBIQUITIN-ACTIVATING ENZYME 1 |
| Molecular Weight(MW) | 120.3 kDa forArabidopsis thaliana117 kDa for Rice |
| Function | UBA1 encodes a ubiquitin-activating enzyme (E1). It is involved in the first step in conjugating multiple ubiquitins to proteins targeted for degradation. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
