WB
Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves
Electrophoresis: 12% SDS‑PAGE
Transfer: NC (nitrocellulose) membrane at 10 V overnight
Blocking: 5% skim milk, incubated at room temperature for 1 h
Primary antibody: diluted 1:5000 and incubated at room temperature for 1 h