WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC membrane at 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted 1:5000, incubated at room temperature for 1 h| Product Name | Anti-FAD2/ Delta(12)-fatty-acid desaturase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT3G12120，P46313 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT3G12120 |
|---|---|
| Alternative Names | ATFAD2 FAD2 FATTY ACID DESATURASE 2 |
| Molecular Weight(MW) | 44 kDa forArabidopsis thaliana |
| Function | Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC membrane at 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
