WB
Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-VHA-C/DET3 V-type proton ATPase subunit C |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G12840, Q9SDS7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT1G12840 |
|---|---|
| Alternative Names | ARABIDOPSIS THALIANA VACUOLAR ATP SYNTHASE SUBUNIT C ATVHA-C DE-ETIOLATED 3 DET3，VATC，VHA-C，V-ATPase subunit C |
| Molecular Weight(MW) | 42.6 kDa forArabidopsis thalianaand Rice |
| Function | DET3 encodes subunit C of the vacuolar H(+)-ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
