WB
Loading: Approximately 3 μg total protein from Arabidopsis and rice leaves
Electrophoresis: 10% SDS‑PAGE
Transfer: NC (nitrocellulose) membrane, 10 V overnight
Blocking: 5% skim milk, incubated at room temperature for 1 h
Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h