WB
Loading: Serially diluted antigen protein;
Approximately 3 μg of total protein from Arabidopsis thaliana leaves
Electrophoresis: 12% SDS-PAGE
Membrane transfer: NC (nitrocellulose) membrane, 10 V overnight
Blocking: 5% skim milk, incubated at room temperature for 1 h
Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h