WB
Loading: Approximately 3 μg of total protein from Arabidopsis thaliana leaves Electrophoresis: 8% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GMII/ Alpha-mannosidase 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G14950，Q9LFR0 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Gene Symbol | AT5G14950 |
|---|---|
| Alternative Names | ATGMII GMII GOLGI ALPHA-MANNOSIDASE II |
| Molecular Weight(MW) | 134.7 kDa forArabidopsis thaliana |
| Function | GMII encodes a golgi alpha-mannosidase, an enzyme responsible for the formation of major complex-type N-glycans. |
WB
Loading: Approximately 3 μg of total protein from Arabidopsis thaliana leaves Electrophoresis: 8% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
