WB
Loading: Approximately 3 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-HSP70-3/ Heat shock 70 kDa protein 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT3G09440，O65719 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT3G09440 |
|---|---|
| Alternative Names | HEAT SHOCK PROTEIN 70-3 HSP70-3 |
| Molecular Weight(MW) | 71 kDa forArabidopsis thaliana |
| Function | Heat shock protein 70 isoform. |
WB
Loading: Approximately 3 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
