WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-HSCB/ Iron-sulfur cluster co-chaperone protein HscB hom |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G06410，Q8L7K4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT5G06410 |
|---|---|
| Alternative Names | HSCB |
| Molecular Weight(MW) | 28.9 / 22.4 kDa forArabidopsis thaliana |
| Function | HscB is a mitochondrial cochaperone involved in [Fe-S] cluster biosynthesis and iron homeostasis. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
