WB
Loading: Approximately 3 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 8% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-FUM1/ Fumarate hydratase 1, mitochondrial |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT2G47510，P93033 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT2G47510 |
|---|---|
| Alternative Names | FUM1 FUMARASE 1 |
| Molecular Weight(MW) | 53 / 48 kDa forArabidopsis thaliana |
| Function | FUM1 encodes a mitochondrial-localized protein. The FUM1 gene appears to be essential, suggesting that FUM1 may play a crucial role as a fumarase in the tricarboxylic acid cycle. |
WB
Loading: Approximately 3 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 8% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
