WB
Loading: Serially diluted peptide antigen Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-HSFA1A/ Heat stress transcription factor A-1a |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated peptide from Arabidopsis thaliana AT4G17750，P41151 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT4G17750 |
|---|---|
| Alternative Names | HSF1 HSF13，HSFA1A，Heat shock factor protein 1，Heat shock transcription factor 1 |
| Molecular Weight(MW) | 55.7 kDa forArabidopsis thaliana |
| Function | Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). |
WB
Loading: Serially diluted peptide antigen Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
