WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-HCF109/ Peptide chain release factor PrfB1, chloroplast |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G36170，Q9LVY0 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Gene Symbol | AT5G36170 |
|---|---|
| Alternative Names | PRFB1，HCF109，RF2，AtPrfB1，AtPrfB，HIGH CHLOROPHYLL FLUORESCENT 109 |
| Molecular Weight(MW) | 51 / 45 kDa forArabidopsis thaliana |
| Function | HCF109 directs the termination of translation in response to the peptide chain termination codon UGA. It is required for the proper translation, stability and normal processing of UGA-containing polycistronic transcripts in chloroplasts. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
