WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana leaves
Electrophoresis: 6% SDS-PAGE
Transfer: PVDF membrane, 25 V for 75 min
Blocking: 5% skim milk, incubated at room temperature for 1 h
Primary antibody: Diluted at 1:1000, incubated at room temperature for 1.5 h