WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GRF6/ 14-3-3-like protein GF14 lambda |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana AT5G10450. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT5G10450，LOC_Os03g50290 |
|---|---|
| Alternative Names | 14-3-3 PROTEIN G-BOX FACTOR14 LAMBDA 14-3-3LAMBDA AFT1 G-BOX REGULATING FACTOR 6 GRF6，AFT1 RCI2 |
| Molecular Weight(MW) | 28 / 28 kDa for Arabidopsis29 / 28 kDa for Rice |
| Function | GRF6 encodes a member of the 14-3-3 gene family that is a lambda isoform (14-3-3λ). It interacts with APX3 (ascorbate peroxidase) and AKR2, suggesting a role in mediating oxidative metabolism in stress response. This protein was shown to colocalize and interact with SERK1 by which it is phosphorylated. |
WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
