WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-SYT1/SYTA/ Synaptotagmin-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT2G20990，Q9SKR2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT2G20990 |
|---|---|
| Alternative Names | ATSYT1 ATSYTA NTMC2T1.1 NTMC2TYPE1.1 SYNAPTOTAGMIN 1 SYNAPTOTAGMIN A SYT1 SYTA |
| Molecular Weight(MW) | 61.7 kDa for Arabidopsis |
| Function | SYT1 encodes a protein specifically localized to the ER-PM boundary with similarity to synaptotagmins, a class of membrane trafficking proteins. SYT1 is expressed in all tissues. It plays an important role in maintaining plasma membrane integrity during freezing and osmotic stresses. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
