WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-SCO1/ Elongation factor G, chloroplastic |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT1G62750，Q9SI75 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Gene Symbol | AT1G62750 |
|---|---|
| Alternative Names | CPEFG，ATSCO1 ATSCO1/CPEF-G SCO1 SNOWY COTYLEDON 1 |
| Molecular Weight(MW) | 86 / 77.3 kDa for Arabidopsis |
| Function | Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
