WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-HEMD/ Uroporphyrinogen-III synthase, chloroplastic |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT2G26540，O48721 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | AT2G26540 |
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| Alternative Names | ATDUF3 ATUROS DOMAIN OF UNKNOWN FUNCTION 724 3 DUF3 HEMD UROPORPHYRINOGEN III SYNTHASE UROS |
| Molecular Weight(MW) | 34 / 26 kDa for Arabidopsis |
| Function | HEMD encodes a uroporphyrinogen-III synthase involved in tetrapyrrole biosynthesis. The protein localizes to the chloroplast. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
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| Form | Liquid |
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| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
