WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-XTH4/ Xyloglucan endotransglucosylase/hydrolase protein |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT2G06850，Q39099 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT2G06850 |
|---|---|
| Alternative Names | At-XTH4 XTH-4，EXGT-A1 EXT |
| Molecular Weight(MW) | 34.3 / 31.5 kDa for Arabidopsis |
| Function | XTH4 catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). It cleaves and religates xyloglucan polymers. It is an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
