WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-HCF101 / Fe-S cluster assembly factor HCF101 |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT3G24430，Q6STH5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | AT3G24430 |
|---|---|
| Alternative Names | HCF101 HIGH-CHLOROPHYLL-FLUORESCENCE 101 |
| Molecular Weight(MW) | 57.8 / 51 kDa for Arabidopsis |
| Function | HCF101 encodes chloroplast protein high chlorophyll fluorescence 101. It serves as a chloroplast scaffold protein that specifically assembles [4Fe-4S] clusters and transfers them to the chloroplast membrane and soluble target proteins. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
