WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-F5H/ Cytochrome P450 84A1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT4G36220，Q42600 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | AT4G36220 |
|---|---|
| Alternative Names | CYP84A1 CYTOCHROME P450 84A1 F5H FAH1 FERULIC ACID 5-HYDROXYLASE 1 |
| Molecular Weight(MW) | 58.7 kDa for Arabidopsis |
| Function | F5H encodes ferulate 5-hydroxylase. It is involved in lignin biosynthesis. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
