WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves, together with serially diluted antigen protein
Electrophoresis: 10% SDS‑PAGE
Transfer: NC (nitrocellulose) membrane, 10 V overnight
Blocking: 5% skim milk, incubated at room temperature for 1 h
Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h