WB
Loading: 3 μg total protein from Arabidopsis thaliana / rice leaves
Electrophoresis: 12.5% SDS-PAGE
Transfer: NC (nitrocellulose) membrane, 10 V overnight
Blocking: 5% skim milk, incubated at room temperature for 1 h
Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h