WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-SAHH1/ Adenosylhomocysteinase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT4G13940，O23255 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT4G13940，LOC_Os11g26850 |
|---|---|
| Alternative Names | SAHH1 EMB1395 HOG1，MEE58 S-ADENOSYL-L-HOMOCYSTEIN HYDROLASE 1 SAH1 |
| Molecular Weight(MW) | 53 / 50 kDa for Arabidopsis56 / 50 kDa for Rice |
| Function | SAHH1 encodes a S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
