WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-UFGT/ Anthocyanidin 3-O-glucoside 2'''-O-xylosyltransfe |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT5G54060，Q9LVW3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | AT5G54060 |
|---|---|
| Alternative Names | UDP-GLUCOSE:FLAVONOID 3-O-GLUCOSYLTRANSFERASE UDP-GLUCOSYL TRANSFERASE 79B1 UF3GT UGT79B1 UFGT |
| Molecular Weight(MW) | 52 kDa for Arabidopsis |
| Function | UFGT contributes to the last few anthocyanin biosynthetic steps. It converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1->2)glucoside. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
