WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 10% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-FUG1/ Translation initiation factor IF-2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT1G17220，Q9SHI1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT1G17220 |
|---|---|
| Alternative Names | FU-GAERI1 FUG1 |
| Molecular Weight(MW) | 109.7 / 103 kDa for Arabidopsis |
| Function | FUG1 encodes a chloroplast localized protein with similarity to translation initiation factor 2. It is one of the essential components for the initiation of protein synthesis. It protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. It is also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 10% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
